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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: J Pathol. 2014 Dec 17;235(4):593–605. doi: 10.1002/path.4486

Figure 4. Effect of FGF9 withdrawal on established lung tumors.

Figure 4

DT mice were fed doxycycline chow for 1-month (n=4), and the presence of tumor nodules in their lungs was confirmed by microCT (A, D and G). Then, they were switched into dox-free chow, and the status of tumor nodules in their lungs was examined monthly. Many of the previously identified nodules persisted, and their size remained the same or increased (compare B and C to A, and E and F to D). Some nodules appeared de novo after doxycycline withdrawal and increased in size between months 3 and 4 (compare H and I to G). The role of signaling through the FGF/FGFR pathway. (J-K) EpCAM+ epithelial cells were sorted from dox-fed, DT mouse lungs and then co-cultured with wt fibroblasts in the 3D-sphere/colony formation assay. Culture wells were treated with the FGFR inhibitor or a vehicle control (n=3 well replicates, two independent experiments). FGFR inhibitor-treated cells formed spheres that were of smaller size and fewer number than those in the control group. (L) Quantification of sphere number and diameter. (*p<0.01, compared to J). Scale bar: (J) 100 μm. See also Supplementary Figures S5–S7.