Figure 1.

Gastroids differentiate and can be successfully infected with Helicobacter pylori. (A) Bright field image of a single gastroid. (B) Cross-sectional image of a single gastroid stained for F-actin (red); nuclei are labelled with Hoechst (blue). (C) Three dimensional reconstruction of a single gastroid stained for F-actin (red) and nuclei (blue). (D–G) Representative staining of gastroids for (D) gastric mucin (red), labelling mucus cells; (E) H⁺K⁺ATPase (red), labelling parietal cells; (F) gastrin (green), labelling G-cells and (G) chromogranin (red), labelling enterochromaffin-like cells. (D–G), nuclei are labelled with Hoechst (blue). (H) Uninfected (left panel) and infected (right panel) gastroids stained for occludin (red) and H. pylori (arrows, green). (I) Gastroids were uninfected, treated with LiCl or infected with H. pylori strain 7.13 (green) and stained for β-catenin (red).