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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Gut. 2014 Aug 14;64(5):720–730. doi: 10.1136/gutjnl-2014-307650

Figure 3.

Figure 3

Helicobacter pylori alters the topography of claudin-7 in gastroids and MKN28 gastric epithelial cells. Immunocytochemical localisation of claudin-7 in uninfected (A) gastroids and (C) MKN28 cells and H. pylori-infected (B) gastroids and (D) MKN28 cells, demonstrating accumulation of claudin-7 in cytosolic vesicles and discontinuous claudin-7 staining at the cell membrane in infected samples. (E and F) MKN28 cells were cocultured for 48 h with H. pylori strain 60190 and claudin-7 levels were determined by western blotting. (E) Representative western blot for claudin-7 or actin in detergent-insoluble and detergent-soluble MKN28 fractions in the presence or absence of H. pylori. (F) Densitometric analysis demonstrating loss of claudin-7 from the insoluble fraction of H. pylori-infected cells. Data are expressed as means±SEM, n=3 independent replicates.

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