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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Mol Microbiol. 2014 Dec 30;95(4):624–644. doi: 10.1111/mmi.12890

Figure 6. mut6-prfA is not impaired in PrfA activity.

Figure 6

A. Growth of WT L. m., L.m. mut6-prfA and ΔprfA bacteria in LB-MOPS-G1P medium. B. PlcA activity in the supernatants of WT L.m., L.m. mut6-prfA, ΔprfA and ΔplcA mutants measured using a PI-PLC phospholipase activity assay. Bacteria were grown in LB-MOPS-G1P medium and supernatants were harvested at mid-exponential growth. Results are average of 3 independent biological repeats. Error bars represent standard error of the mean. C. Analysis of LLO activity in the supernatants of WT L.m., L.m. mut6-prfA, ΔprfA and Δhly mutants using hemolysis assay in red blood cells. Bacteria were grown in LB-MOPS-G1P medium and supernatants were harvested at mid-exponential growth.