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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Mol Microbiol. 2014 Dec 30;95(4):624–644. doi: 10.1111/mmi.12890

Figure 8. CodY and its prfA-intragenic binding site play a major role in L. monocytogenes virulence.

Figure 8

A. Intracellular growth of WT L. m., ΔcodY, L.m. mut6-prfA and L.m. CodY R61A bacteria in BMD macrophages. Results are representative of 3 independent biological repeats. Error bars represent the standard error of the mean. B. RT-qPCR analysis of prfA during intracellular growth of WT L. m., ΔcodY and L.m. mut6-prfA bacteria in bone marrow derived (BMD) macrophages. Results are average of 3 independent biological repeats. Transcription levels are represented as relative quantity (RQ), relative to levels in WT L.m bacteria grown to mid-log in BHI rich medium. Error bars represent 95% confidence interval, * represents P value < 0.05. C. Intravenous infection of C57BL/6 mice with WT L. m., ΔcodY, L.m. mut6-prfA and L. m. CodY R61A bacteria. Bacterial colony forming units (CFUs) were numerated at 72 h.p.i. from livers and spleens taken from 10 infected mice for each strain. The results are mean of 2 independent experiments in which 5 mice were infected in each group. Mann-Whitney tests were performed for statistical significance (* P value < 0.05).