Figure 1.

Experimental design. Assessment of neurological function was performed before (pre), and at day (d)1, 2, 7 and 14 following experimental stroke induced by photothrombosis (PT) (study I); 2-photon-laser-microscopy (2PLSM) was performed before and on days 3, 7 and 14 after PT (study II). One group of animals was treated with AMD3100 (0.5 mg/kg, twice daily), and control animals received intraperitoneal saline injections for the same time (vehicle (VH) twice daily). At the endpoint of the study (day 14), animals were sacrificed and brains processed for endpoint analyses. Fractalkine and cytokine levels were determined in CX₃CR1 wild-type, heterozygote and knockout mice, respectively, on day 6 after PT (study III).