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. 2015 Feb 7;12:24. doi: 10.1186/s12974-014-0232-1

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© Walter et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Illustration of 2-photon-laser-microscopy image (2PLSM) analysis. In the cartoon to the left the red circle represents the photothrombotic stroke, and the green square the thinned skull-window, through which green fluorescent protein (GFP)-CX₃CR1⁺ cells were imaged. Close to the infarct we defined region A, representing the peri-infarct zone. The opposite side of the cranial window was defined as region B.