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. 2015 Feb 7;12:24. doi: 10.1186/s12974-014-0232-1

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© Walter et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phenotype of green fluorescent protein (GFP)-positive cells in the ischemic territory. Upper panels: colocalization of CD68 (Cy3, red), GFP (green, CX₃CR1) and CD45 (white, Cy5) in the ischemic territory of a vehicle- and AMD3100-treated mouse after photothrombosis (PT). Middle panels illustrates the expression of ionized calcium binding adaptor molecule 1 (Iba1) in CD68/GFP- and CD45/GFP-positive cells. Scale bar: 50 μm. Lower panel: colocalization of GFP (green) with Iba1 (Cy3, red) in the ischemic core after PT. Scale bar: 10 μm.