Fig. 5.
Western blot analysis to detect the presence of Strep-tag II on purified asialo-rhuEPOP. Lanes 1–3: 10 ng of JMJD2E+Strep-tag II, 35 ng of purified asialo-rhuEPOP and 10 ng of rhuEPOM. The blot was first probed with anti-Strep-tag II antibody (upper panel), followed by stripping and re-probing with anti-EPO antibody (lower panel).