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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Plant Cell Rep. 2014 Dec 14;34(3):507–516. doi: 10.1007/s00299-014-1730-4

Fig. 7.

Fig. 7

Western blot analysis to detect stability of asialo-rhuEPOP in crude protein extracts made with acetate buffer (pH 5). Crude protein extracts of A56-5 transgenic line (containing 35 μg proteins) with and without protease inhibitors (PI) were incubated at 4°C for indicated interval of time. The samples were then boiled with 4X SDS-PAGE sample buffer. These together with total protein extract (TPE) and rhuEPOM (positive control, 10 ng) were applied onto a 12.5% SDS-PAGE gel. The blot was probed with mouse anti-EPO antibody.