Skip to main content
. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Pharm Res. 2014 Sep 16;32(3):1028–1044. doi: 10.1007/s11095-014-1515-z

Fig.10.

Fig.10

Effect of peripherally cross-linking the shell of F127 PM on tumor vascular shutdown and subsequent growth inhibition by physically loaded CA4 after IV administration. F127 polymer micelles alone (F127 PM) or individually cross-linked with ED at 76% of total PEO blocks (X-F127 PM) were loaded with CA4 at 22.9 wt% as described in Fig.5. 4T1 cells stably expressing luciferase (4T1-Luc) were injected SQ into the mammary fat pad of female BALB/c mice and grown until ~100 mm3 before treatment. (A) On the day of treatment, an average luciferase signal was obtained from the primary tumors before PBS, water-soluble CA4P, or CA4 loaded in F127 PM or X-F127 PM was injected IV (1 mg / kg). The average percent decrease in the initial luciferase signal ± SD (n = 6 mice) (% Vascular Shutdown) was then compared to PBS at the same time point by Kruskal-Wallis nonparametric ANOVA with Dunn’s post-test (aP < 0.05; bP < 0.01; cP < 0.0001). (B) Average tumor volumes ± SEM (n = 6 mice) after treatment with CA4P (closed circles), CA4 in F127 PM (closed squares), or CA4 in X-F127 PM (cross-hatched squares) were compared to PBS (open circles) on Day 5 post-CA4 injection by Kruskal-Wallis nonparametric ANOVA with Dunn’s post-test (**P < 0.01).