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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Pharm Res. 2014 Sep 16;32(3):1028–1044. doi: 10.1007/s11095-014-1515-z

Fig.11.

Fig.11

Effect of peripherally cross-linking the shell of F127 PM on the distribution of physically loaded CA4 after IV administration in tumor-bearing mice. Uncross-linked F127 polymer micelles (F127 PM) or F127 PM individually cross-linked with ED at 76% of total PEO blocks (X-F127 PM) were loaded with CA4 at 22.9 wt% as described in Fig.5. 4T1 cells stably expressing luciferase (4T1-Luc) were injected SQ into the mammary fat pad of female BALB/c mice and grown until ~100 mm3 before treatment. Water-soluble CA4P (open bars) or CA4 loaded in F127 PM (grey bars) or X-F127 PM (closed bars) was then injected IV (1 mg / kg) and the mass of CA4 / g tissue was determined by LC-MS/MS. (A) Average percent of injected dose ± SEM (n = 6 mice / time point) was determined from the mass of CA4 / g tissue and compared to CA4P at the same time point by Kruskal-Wallis nonparametric ANOVA with Dunn’s post-test (*P < 0.05; **P < 0.01; ***P < 0.001). (B) Average issue to plasma ratios ± propagated SEM were calculated from data in A and drug plasma concentrations at the indicated time point from PK data in Table 1 and compared to CA4P at the same time point by one way ANOVA with Tukey’s post-test (*P < 0.05; **P < 0.01; ***P < 0.001). n.d. – not detected.