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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Pharm Res. 2014 Sep 16;32(3):1028–1044. doi: 10.1007/s11095-014-1515-z

Fig.8.

Fig.8

Effect of peripherally cross-linking the shell of F127 PM on the activity of physically loaded CA4 against the well resistance of proliferating HUVEC monitored by electric cell impedance substrate (ECIS). CA4 was solubilized with 0.25% v/v DMSO or loaded in uncross-linked F127 polymer micelles (F127 PM) or F127 PM cross-linked with ED at 76% of total PEO blocks (X-F127 PM) at 22.9 wt% CA4 as described in Fig.5. Subconfluent HUVEC at densities equivalent to IC50 studies in Fig.7 were grown for 24 h and treated for 4 h (“Rx”) with growth media (Media), empty F127 PM (0.1 µg/mL), empty X-F127 PM (0.1 µg/mL), or CA4 in the indicated formulation. Average well resistances (n=2 on each array) were normalized by dividing the resistance from treated wells by the resistance from wells with growth media alone using ECIS software.