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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Nat Protoc. 2013 Feb 14;8(3):525–538. doi: 10.1038/nprot.2013.016

Figure 2. Custom fabricated flow cell that contains channels for laminar buffer flow and a flow-free reservoir.

Figure 2

(a) Flow cell pattern showing the four input channels (1–4) that converge into a common channel. A reservoir that has its own independent inlet is connected to the common flow channel by a small (100 μm wide) passage; scale bar = 10 mm (b) Photograph of the top of the assembled flow cell. (c) Photograph of the bottom of a flow cell showing SM buffer, dyed either with red or with green food coloring, pumped through channels 1–4. The inset shows a magnified view of the common channel and reservoir. Even though no physical boundary exists, there is no mixing between fluids flowing from the four input channels after their convergence into the single common channel. Diffusion of the solution components from channel 4 into the reaction reservoir is limited to the narrow connecting passage.