Fig. 7.

Polyhedra production (per ml) by Sf9 cultures supplemented with chromatographic fractions 50 min before baculovirus infection. All cultures were infected with baculovirus 48 h after the start of the cultures at an MOI of 1. Kinetics of the concentration of polyhedra after infection of Sf9 cells grown in Grace medium with 10 % (v/v) FBS with baculovirus in the presence or absence of different HB fractions. Control Grace medium with 10 % (v/v) FBS. Fraction Q2–5 Control supplemented with 3 % (v/v) of fraction Q2–5. Fraction Q7–14 Control supplemented with 3 % (v/v) of fraction Q7–14. Fraction Q16–19 Control supplemented with 3 % (v/v) of fraction Q16–19. Pool 01 Control supplemented with 3 % (v/v) of fraction Pool 01. Tris Control supplemented with 3 % (v/v) of buffer Tris 20 mM, NaCl 1 M. For all conditions, Sf9 cells were supplemented 50 min before infection with baculovirus