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Transcriptional level of GRP78, XBP1 and PDI of recombinant CHO cells addressed by qPCR. Values were calculated relative to beta-actin housekeeping gene and normalized. Formula used for calculation: ratio = [ECq(ref)(ref)]:[ECq(target)(target)]. None of the ER-stress related proteins exceeded the values of the host CHO DG44 cells, indicating there is no ER-stress present in the recombinant cell lines
