Table 2.
Cell culture performance and intracellular HC/LC analysis
| μ (d−1) | qp (pg c−1 d−1) | vol. Titer (mg l−1) | MFI | ||
|---|---|---|---|---|---|
| HC | LC | ||||
| CHO/IgM-617 | 0.50 (±0.12) | 25.00 (±5.15) | 75.25 (±25.64) | 27.14 (±6.96) | 16.45 (±4.02) |
| CHO/IgM-012 | 0.51 (±0.10) | 3.59 (±0.81) | 9.94 (±3.51) | 28.31 (±3.34) | 16.10 (±2.62) |
| HEK/IgM-617 | 0.34 (±0.09) | 4.60 (±1.71) | 23.44 (±11.28) | 23.75 (±1.35) | 52.97 (±7.19) |
| HEK/IgM-012 | 0.33 (±0.08) | 0.21 (±0.08) | 0.92 (±0.31) | 10.86 (±0.17) | 34.61 (±2.00) |
Standard deviation in brackets represent seven consecutive passages (for μ, qp and vol titer) and three biological samples for flow cytometry analysis (MFI)