Fig. 3.

Somatic metaphases with C-banding of Microglanis cottoides: (a–b) diploid male, (c–d) diploid female and (e–g) triploid female and Pseudopimelodus pulcher (h–i). C-banding stained with Giemsa (a, c, e, f, h) and overlapping DAPI/CMA₃ (b, d, g). The arrowheads in cells of M. cottoides (a–g) indicate the NOR-bearing pairs, and the arrows indicate the two chromosomes with the heterochromatic block in the diploid (c–d) and triploid (f–g) females; in the diploid male (a–b), the arrows indicate chromosome pair one without the heterochromatin block. In (e), the asterisk indicates the two supernumerary chromosomes that are totally heterochromatic; in (h), the asterisk indicates the heterochromatic chromosome pair with AT and GC sites in the pericentromeric region and GC in the terminal region. The scale bar represents 5 μm