Table 1.

Growth media and detection reagents used for substrate degradation assays.

Test substrate	Growth medium	Detection reagent
Starch	0.8 % peptone; 0.5 % NaCl; 0.6 % sol. starch; 1.2 % agar; H2O	Lugol’s solution
Chitin	0.8 % peptone; 0.5 % NaCl; 0.6 % colloidal chitin; 1.2 % agar; H2O	none
Cellulose	0.8 % peptone; 0.5 % NaCl; 0.6 % carboxymethyl cellulose; 1.2 % agar; H2O	Congo red; 0,5 M HCl
Peptides and protein	0.8 % nutrient broth; 1.3 % powdered milk; 1.2 % agar; H2O ; pH 7.2	none
Lipid	0.8 % peptone; 0.5 % NaCl; 1 % Tween 80; 1.2 % agar; H2O	none
Lignin	1 % malt extract; 0.4 % yeast extract; 0.4 % glucose ; 1.2 % agar; H2O	0.2 % K2HPO4; 0.2 % KCl; 0.2 % MgSO4·7 H2O; 2 % agar; ABTS 1 mM; H2O; pH 5