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. 2014 Nov 26;113(4):1165–1174. doi: 10.1152/jn.00614.2014

Fig. 2.

Fig. 2.

Effects of the nicotine analog cytisine on mEPSC frequency and EPSCs evoked after tractus solitarius stimulation in GABAergic NTS neurons. A: traces showing the effect of bath-applied cytisine (100 μM) on mEPSCs in GFP-positive mNTS neurons. Left: mEPSCs in control ACSF containing TTX (2 μM). Center: the same neuron in the presence of cytisine. Right: 15 min after washout of cytisine. Arrows point to temporally expanded 1-s periods from the respective traces above. B: cumulative probability plot of mEPSC frequency in the 3 conditions (control, cytisine, wash) from the recording in A. C: plots showing mean the effect of cytisine on mEPSC frequency (left) and amplitude (right) in GABAergic NTS neurons (n = 8). D: evoked (e)EPSC responses in a GABAergic NTS neuron to paired stimulation of tractus solitarius. Left: averaged traces showing the response of a single neuron in control ACSF and in the presence of cytisine. Although cytisine tended to diminish the amplitude of the 1st eEPSC and increase the amplitude of the 2nd eEPSC, amplitude changes were not significant (P > 0.05). Right: the same responses, with the 1st EPSC amplitude normalized to that recorded in ACSF, demonstrate the larger 2nd response, relative to the 1st, in the presence of cytisine. Averages of 25–30 consecutive responses are shown for each condition. E: plot showing mean change in paired-pulse response ratio (PPr) in the presence of cytisine for the 4 GABAergic NTS neurons. *Significant increase in mEPSC frequency and PPr in the presence of cytisine (P < 0.05).