Table 2. Effect of Chondrogenic Medium and Microencapsulation on ASCs from Multiple Donors.
| CM/GM | ||||
|---|---|---|---|---|
| wk1 TCPS | wk1 μB | wk2 TCPS | wk2 μB | |
| mRNA Levels | ||||
| Acan/Rps18 | *94.5±41.3 | *9.9±4.0 | *184.7±95.6 | *18.3±5.6 |
| Col2/Rps18 | 3.6±1.4 | 2.4±1.5 | 2.5±0.8 | 10.0±4.5 |
| Col10/Rps18 | *48.8±33.8 | *14.9±4.2 | *11.7±7.9 | *23.4±11.2 |
| Comp/Rps18 | *8.7±2.1 | *4.1±1.0 | *8.0±1.9 | *8.2±2.4 |
| Igf1/Rps18 | *2.8±0.8 | 1.6±0.6 | 4.6±1.3 | *3.3±0.6 |
| Pthlh/Rps18 | *0.6±0.2 | *0.1±0.0 | 0.5±0.1 | *0.1±0.0 |
| Sox9/Rps18 | 1.8±0.3 | *1.9±.3 | *2.2±0.6 | *2.4±0.2 |
| Tgfb1/Rps18 | *2.0±0.2 | *1.5±.4 | *1.6±0.2 | *1.0±0.2 |
| Tgfb2/Rps18 | *1.4±0.3 | *1.0±0.5 | 1.5±0.3 | 1.1±0.3 |
| Tgfb3/Rps18 | *8.4±1.8 | 0.6±.2 | *5.0±1.2 | 0.6±0.2 |
| Growth Factor Secretion | ||||
| IGF-I/DNA | *4.7±2.4 | 1.9±0.3 | 6.0±2.9 | 2.0±0.7 |
| TGF-β3/DNA | 1.2±0.1 | 1.0±0.1 | 1.1±0.2 | 1.0±0.3 |
| μB/TCPS | ||||
| wk1 GM | wk1 CM | wk2 GM | wk2 CM | |
| mRNA Levels | ||||
| Fgf2/Rps18 | *0.3±0.1 | *.05±0.1 | 0.6±0.3 | 0.7±0.3 |
| Nog/Rps18 | 1.1±0.6 | *0.1±0.0 | 1.4±0.7 | *0.2±0.1 |
| Tgfb1/Rps18 | 1.9±0.3 | 1.5±0.4 | 1.8±0.3 | 1.0±0.2 |
| Tgfb2/Rps18 | 6.1±1.7 | 2.0±0.5 | 2.4±0.6 | 1.6±0.3 |
| Vegfa/Rps18 | 2.2±0.7 | 0.9±0.3 | 2.0±0.7 | 1.3±0.5 |
| Growth Factor Secretion | ||||
| FGF-2/DNA | 0.8±0.4 | 1.6±1.4 | *6.1±2.3 | 5.0±2.4 |
| VEGF-A/DNA | 0.5±0.2 | 1.1±0.7 | 1.3±0.5 | 2.1±1.1 |