Table 2.

Primers used for quantitative PCR quantification of target mRNAs and for pDrive plasmids

Species/Gene	Forward (5′-3')	Reverse (5′-3')	Tann, °C	Source
Zebrafish (Danio rerio)
COX4-1	CAAGTTTGTGCAGCAGCTG	CAAAGAAGAAGATTCCTGCAA	63	29
COX4-2	CGACAGACCTTACAAAGACATCC	GAAGAATAAGATCCCAGCCGT	63	29
IGFBP-1	ACGCAAGAAACTGGTGGAAC	GGGCTGTCTGGAGTTCGATA	59	30
VegFA	AGTATCCCGATGAGATCGAGCACA	CACCTCCATAGTGACGTTTCGTGT	59	—
β-Actin†	TCCAGGCTGTGCTGTCCCTGTA	GTCAGGATCTTCATGAGGTAGTC	59	12
RPL13A	TCTGGAGGACTGTAAGAGGTATGC	AGACGCACAATCTTGAGAGCAG	59	40
Goldfish (Carassius auratus)
COX4-1	AGCTTCGCTGAAATGGAAA	CCTCATGTCCAGCATCCTCT	59	14
COX4-2	AAACTTGCCTTGTACAGGCTTACG	AAGAAGAAGATGCCGCCCAC	56	—
IGFBP-1	TGGTTGGGCAGGAACCAATC	TCTCGAAGCACCTCCTCACA	59	—
EF-1α	CAGGTCATCATCCTGAACCAC	AACGACGGTCGATCTTCTC	59	5
TBP	AGTGGTGCAGAAGTTGGGTTTTCC	ATGTGSSGCACCAGGCCCTCTAA	59	12
Rainbow trout (Oncorhynchus mykiss)
COX4-1*	CACATGGTGTTGCCAAGGTAGAC	GCGCTCAGCTCTGTCACAAACT	63	—
COX4-2*	ACCACGAGGTGTCCGACTCA	AGGACGTCCTTCCATGGTCTGT	63	—
VegFA	AGACAGCCCACATACCCAAG	AAGACGTCCACCAGCATCTC	59	—
EF-1α	TCCTCTTGGTCGTTTCGCTG	ACCCGAGGGACATCCTGTG	59	—
Tilapia (Oreochromis niloticus)
COX4-1	GCAACCAGAGCCCTAAGCCTTATT	CTCTGCACTCAGGTTCTGCACA	67	—
COX4-2	GCTGAGGTAGCACAGTCTGGA	TTCCCTAGAGAGGCTCCATCTTTC	67	—
IGFBP-1	AGGAGAGCATGAAAGCCAAA	TCCGTCCAGAGAGGATTCAC	59	—
Human (Homo sapiens)
COX4-1	GAGCAATTTCCACCTCTGT	CAGGAGGCCTTCTCCTTCTC	59	14
COX4-2	ATTTCCTCCAAAGCCGATCAC	GAGACAGCTGGGGATGCAAGTCA	65	—
VegFA	AGCTACTGCCATCCAATCGAGA	TACACGTCTGCATGGTGATGTTGG	59	—
EF-1α‡	ATTGATGCTCCTGGACACAGA	CAGCAACAATCAGGACAGCACAGT	59	—
Mouse (Mus musculus)
COX4-1	GCAGCCTTTCCAGGGATG	GCTCTTCTCCCAAATCAGA	59	—
COX4-2	AGAACTAGAGGGACAGGGACACA	TCGCTGAGCTCTGTGCAGAA	59	—
VegFA	CCAAGGCCAGCAAATCCCTGTGGGCC	CCGCCTCGGCTTGTCACA	59	8
β-Actin	AGCACCATGAAGATCAAGATCAT	ATCCACATCTGCTGGAAGGT	59	27
Anole (Anolis carolinensis)
COX4-1	ATGGAGTGCTCTTTCGCTGG	AAAGCCACTGAGGCCAATGA	59	—
COX4-2	AGTTATCCGCTGCCAGACAC	ACGGTCTTCCATTCGTTGCT	59	—
VegFA§	TTCACAGACTCACGTTGCAAGTCG	GCAAGTGCGCTCGTTTAACTCAAG	59	—
Turtle (Chrysemys picta bellii)
COX4-1*	CCAGAGAATGCTGGATATGA	CCATTCCTTCTTTTCGTAGTCCCA	59	—
COX4-2	AAGAGAAGGAGAAGGGCTCATGGAAG	TTCGTTGGATGGCCTGTTCATCTC	59	—
pDrive-specific	CCGGGTTGGACTCAAGACGATAGTTA	ACGCTGTAGGTATCTCAGTTCGGT	59	—

T_ann, annealing temperature; see text for additional definitions.

^*Primers were designed from consensus sequences from closely related species.

^†Primer set was used for all other species listed except mouse.

^‡Primer set was used for mouse, anole, and turtle as well.

^§Primer set was used for anole and turtle.