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YAP/TAZ siRNA (YAPi/TAZi) attenuates YAP/TAZ message (relative mRNA) and protein levels (Western blot) in IMR-90 fibroblasts. A: YAP and TAZ transcript levels measured by qPCR, normalized to GAPDH, and to 25-kPa control samples. B: Western blotting with mouse anti-YAP (63.7) antibody (Santa Cruz Biotechnology) detects YAP (65 kDa) and TAZ (45 kDa) bands in siCTRL but not YAPi/TAZi samples.
