Table 5.
RQ values from qRT-PCR analysis of isolated primary cardiac cell cytokine expression
| Gene Name | Vehicle | Iso | AG1478 | AG1478 + Iso |
|---|---|---|---|---|
| Cardiomyocyte | ||||
| n | 4 | 8 | 4 | 7 |
| Il1b | 1.00 ± 0.03 | 1.95 ± 0.23* | 0.81 ± 0.12 | 1.92 ± 0.24* |
| Il6 | 1.00 ± 0.16 | 2.92 ± 0.55* | 1.28 ± 0.23 | 3.44 ± 0.89* |
| Tnfa | 1.00 ± 0.16 | 0.58 ± 0.22* | 1.12 ± 0.07 | 1.21 ± 0.07‡ |
| Cardiac Fibroblast | ||||
| n | 5 | 6 | 4 | 5 |
| Ccl2 | 1.00 ± 0.20 | 11.51 ± 4.02* | 0.77 ± 0.26 | 1.68 ± 0.21‡ |
| Ccl6 | 1.00 ± 0.18 | 3.88 ± 1.31* | 1.51 ± 0.28 | 4.03 ± 0.82* |
| Ccl20 | 1.00 ± 0.17 | 1.92 ± 0.28* | 0.61 ± 0.07 | 0.97 ± 0.14‡ |
| Il1b | 1.00 ± 0.49 | 48.49 ± 24.55* | 1.34 ± 0.32 | 44.66 ± 18.03* |
| Il6 | 1.00 ± 0.17 | 9.50 ± 3.30* | 0.73 ± 0.11 | 8.78 ± 2.25* |
| Il17a | 1.00 ± 0.28 | 0.38 ± 0.09* | 0.84 ± 0.19 | 3.06 ± 1.14*‡ |
| Tnfa | 1.00 ± 0.11 | 9.17 ± 1.86* | 1.16 ± 0.20 | 2.63 ± 0.35‡ |
| Tnfsf11 | 1.00 ± 0.34 | 3.86 ± 0.64* | 0.99 ± 0.34 | 0.87 ± 0.41‡ |
Values are relative quantity (RQ) ± SE.
*
P < 0.05 vs. vehicle;
‡
P < 0.05 vs. Iso, one-way ANOVA with Newman-Keuls multiple comparison test.