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. 2015 Feb 1;5(4):399–417. doi: 10.7150/thno.10351

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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qPCR analysis of the transcription of the ES-GFP plasmid and the effect on VEGF or caspase-3. The endostatin (A), VEGF (B) and caspase-3 (C) mRNA levels were measured by qPCR 48 h after UTMD-mediated ES gene delivery. Endostatin mRNA and caspase-3 mRNA levels were significantly higher with CMB delivery of the ES gene compared with NMB and the control group, and the highest levels were observed for CMB105 delivery. For VEGF mRNA, the levels were significantly lower for CMB delivery of the ES gene compared with NMB and the control group, and the lowest levels were observed with CMB105 delivery (^*P<0.01 and ^#P<0.05 compared with control, NMB and CMB105, ^**P<0.001 and ^##P<0.01 compared with control and NMB, n=6 per group).