Skip to main content
. 2015 Feb 1;5(4):399–417. doi: 10.7150/thno.10351

Figure 8.

Figure 8

UTMD-mediated ES-GFP gene transfection in cell culture. (A) GFP expression in HUVECs observed using a laser confocal microscope 24 h post-transfection (20× objective). (B) Typical flow cytometry images for quantitative analysis of transfection efficiency 24 h and 48 h post-transfection. The percentage of GFP-positive cells was determined as the gene transfection efficiency. (C) Analysis of gene transfection efficiency. The transfection efficiency of CMB105 after 24 h was 24.7-fold higher compared with NMB and 1.47-fold higher compared with CMB (*P<0.01 compared with CMB, **P<0.001 compared with NMB). The difference between 24 h and 48 h in each group was not significant (#P>0.05). n=3 per group.