Table 2. Confirmation of C57BL/6NJ-specific SNPs by direct sequencing of PCR products.
| Chromosome | Number of SNPs selected |
Number of SNPs sequenced |
Direct sequencing succeeded |
Confirmed | Data discrepancy |
|---|---|---|---|---|---|
| 1 | 91 | 91 | 67 | 60 | 7 |
| 2 | 98 | 98 | 67 | 61 | 6 |
| 3 | 89 | 45 | 28 | 25 | 3 |
| 4 | 74 | 18 | 8 | 8 | 0 |
| 5 | 64 | 16 | 13 | 11 | 2 |
| 6 | 71 | 48 | 21 | 10 | 11 |
| 7 | 57 | 16 | 9 | 9 | 0 |
| 8 | 82 | 13 | 9 | 8 | 1 |
| 9 | 68 | 12 | 8 | 8 | 0 |
| 10 | 61 | 14 | 10 | 8 | 2 |
| 11 | 50 | 11 | 8 | 8 | 0 |
| 12 | 80 | 12 | 7 | 7 | 0 |
| 13 | 85 | 13 | 9 | 8 | 1 |
| 14 | 54 | 12 | 9 | 8 | 1 |
| 15 | 59 | 11 | 8 | 8 | 0 |
| 16 | 69 | 11 | 7 | 7 | 0 |
| 17 | 62 | 10 | 4 | 4 | 0 |
| 18 | 67 | 10 | 8 | 8 | 0 |
| 19 | 20 | 9 | 5 | 4 | 1 |
| X | 60 | 16 | 10 | 7 | 3 |
| Total | 1361 | 486 | 315 | 277 | 38 |
SNP: single nucleotide polymorphism.