Figure 3. Gene amplification of PDGFRα in glioma cell lines.

(a) Increased Pdgfra allele copy numbers detected by qPCR in 9 cell lines derived from glioma-bearing brains, but not in control cell lines. PCR primers were either generic for all Pdgfra alleles or specific for Pdgfra J/K mutant alleles. Asterisks denote cell lines in which both mutant and wild-type allele amplifications have occurred. (b) Amplification can also include the adjacent genes Kit (co-amplified in 5 out of 9 lines) and Kdr (in 1 out of 9 lines). Arrowheads or # signs denote co-amplification of Kit or Kdr.