Figure 4.

A cysteine-rich peptide (CRP) gene expressed in the chalazal endosperm is strongly repressed in the dash mutant seeds.(a) List of the most down-regulated and up-regulated genes in 8 DAP dash pods compared with WT. Tissue expression data from the Medicago truncatula Gene Expression Atlas were added.(b) Down-regulation of the Mtr.21576.1.S1_at and Mtr.1254.1.S1_at probes set (CRP genes Medtr8g046040 and Medtr2g076980 respectively) in dash seeds compared with WT by qRT-PCR. ACTIN PDF2 and GAPDH were used as reference genes for expression levels normalization.(c) In situ hybridization of the CRP gene Medtr8g046040 in the chalazal endosperm (arrowheads) of WT 12 DAP seeds. Hybridization with the control sense probe (right) did not yield to any signal.