FIGURE 2.

BODIPY-diacylglycerol (DAG) production in Arabidopsis seedlings treated with Al for different times. Seven-day-old WT and NPC4-overexpressing seedlings were treated with 10 μM AlCl₃ for different time intervals (0, 6, and 12 h) and then incubated with BODIPY-phosphatidylcholine (PC) for 2 h. Lipids were extracted at the time intervals indicated, separated by high-performance thin layer chromatography and quantified. Each value is related to the control non-treated cells (100%). The plotted values are the means + SEM from three independent experiments with parallel samples. NPC, non-specific phospholipase C.