FIGURE 6.

Effect of Al on the growth rate of tobacco pollen tubes transiently expressing AtNPC4:YFP. Tobacco pollen was sown on solid germination medium and transiently transformed with AtNPC4:YFP by particle bombardment. After 6 h of germination, pollen tubes were incubated with 50 μM AlCl₃ for additional 2 h. Mean growth rate of pollen tubes expressing AtNPC4:YFP or vector-only control was evaluated using a fluorescence microscope. Data shown are from two independent experiments and represent means + SEM. At least 21 pollen tubes were measured for each variant. NPC, non-specific phospholipase C.