Fig. 3.

ERp29 over-expression “turns-off” activated Wnt/β-catenin signaling. In mesenchymal MDA-MB-231 cells, high expression of nuclear β-catenin activates its downstream signaling involved in cell cycles and cancer stem cell self-renewal. When ERp29 is over-expressed in this cell model, nuclear β-catenin is relocated at the membrane where it binds to E-cadherin, and Wnt/β-catenin signaling is switched off. Meanwhile, over-expression of ERp29 results in up-regulation of TCF3 and increases expression of genes involved in differentiation. N: Nucleus.