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. Author manuscript; available in PMC: 2015 Feb 16.

Published in final edited form as: Cancer Res. 2007 Dec 15;67(24):11687–11695. doi: 10.1158/0008-5472.CAN-07-1406

Irradiation-induced secreted cytokines enhance MSC engraftment. A, MSC were exposed to conditioned supernatant from 4T1 cells treated with 1, 2, or 5 Gy in a transwell migration assay. Migrated cells were fixed, stained, and counted. Columns, mean of triplicate experiments; bars, SE. B, ELISA assays for TGF-β, VEGF, and PDGF-BB were performed on conditioned media from cultured 4T1 cells taken over 48 h. Points, mean taken and tested in triplicate; bars, SE. C, representative tumor sections from murine 4T1 tumors were stained for chemoattractant mediators. The expressions of PDGF-BB and TGF-β after five fractions of 2 Gy, and VEGF expression after a single 2 Gy fraction.

Irradiation-induced secreted cytokines enhance MSC engraftment. A, MSC were exposed to conditioned supernatant from 4T1 cells treated with 1, 2, or 5 Gy in a transwell migration assay. Migrated cells were fixed, stained, and counted. Columns, mean of triplicate experiments; bars, SE. B, ELISA assays for TGF-β, VEGF, and PDGF-BB were performed on conditioned media from cultured 4T1 cells taken over 48 h. Points, mean taken and tested in triplicate; bars, SE. C, representative tumor sections from murine 4T1 tumors were stained for chemoattractant mediators. The expressions of PDGF-BB and TGF-β after five fractions of 2 Gy, and VEGF expression after a single 2 Gy fraction.