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. 2015 Feb 16;7:7. doi: 10.3389/fnagi.2015.00007

Figure 7.

Figure 7

TGF-β1 inhibitors in NIHL treatment. (A) Chronogram of the experiment. Mice were exposed for 30 min to VS noise at 105 dB SPL and after 2 days they were operated on to deliver P17, P144 or saline (n = 6 mice per group) to the inner ear through a bullostomy. ABR tests were performed before and 1, 14 and 28 days after noise exposure. (B) Evolution of threshold of click-evoked ABR in mice exposed to 105 VS2–20 noise for 30 min and treated locally with a single dose of TGF-β1 inhibitors P17 (orange line), P144 (pink) or saline (black), compared to non-exposed (green) and sham-operated (purple) mice . Noise induced notable TS in the first day after damage without noticeable recovery, compared to non-exposed controls (***p ≤ 0.001). No significant differences were observed between mice treated with TGF-β1 inhibitors and those treated with saline. No statistically significant increase in click-evoked ABR threshold was observed in sham-operated mice. (C) Outer hair cell density (mean ± SEM, expressed as %) in mice treated with TGF-β1 inhibitors compared to saline expressed as %) in mice treated with TGF-β1 inhibitors compared to saline treated mice, in the three standard regions (apex, 5–25; middle, 30–55; base, 60–80) at the end of the experiment. No significant differences were observed between mice treated with P17 (orange bars) or P144 (pink) and those treated with saline (white) after exposure.