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. 2015 Feb 16;6:62. doi: 10.3389/fmicb.2015.00062

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Copyright © 2015 Calvo and Cary.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Schematic representation of the A. flavus asparasone gene cluster. The cluster is composed of four genes [putative Gal4-type transcription factor (ZnF); polyketide synthase (PKS); putative high-affinity glucose transporter (MFS1); and a second putative MFS transporter (MFS2)]. The two hypothetical protein encoding non-cluster genes (Pro) flanking the asparasone cluster are shown in white. The size in bp of intergenic regions are shown. Arrowheads denote direction of transcription. (B) Microscopic examination of sclerotia. Sclerotium production in Af70 pyrG-1 (WT) and an Af70 Δpks27 mutant was observed using an Olympus SZH10 stereomicroscope and Nikon DS-Qi1 camera. Panel: (1) colony surface, X10 magnification; (2) colony surface, X25 magnification. (C) Chemical structure of asparasone A. Adapted and modified from Cary et al. (2014).