Table 1.
Primers used for real-time quantitative PCR experiments.
| Target | NM | Forward primer | Reverse primer |
|---|---|---|---|
| OCT3 (pluripotency) | NM_001173531.1 | gtggtcagccaactcgtca | ccaaaaaccctggcacaaact |
| OCT4 (pluripotency) | NM_002701.3 | cctcacttcactgcactgta | caggttttctttccctagct |
| NANOG (pluripotency) | NM_024865 | tggacactggctgaatccttc | cgttgattaggctccaaccat |
| RUNX2 | NM_001024630.3 | agtggacgaggcaagagtttc | gttcccgaggtccatctactg |
| ALP | NM_000478.4 | gatacaagcactcccacttcatctg | ctgttcagctcgtactgcatgtc |
| BGLAP | NM_199173 | ggcgctacctgtatcaatgg | gtggtcagccaactcgtca |
| COL1A1 | NM_000088.3 | gggccaagacgaagacat | caacacccttgccgttgtcg |
| DLX5 | NM_005221 | accagccagaagaagtgac | ccttctctgtaatgcggcca |
| CD105 (ENG) | NM_001144950 | tgcacttggcctacaattcca | agctgcccactcaaggatct |
| ACTB (endogenous control) | NM_001101 | tgaagtgtgacgtggacatc | ggaggagcaatgatcttgat |
| TBP (endogenous control) | NM_001172085 | gtgacccagcatcactgtttc | gcaaaccagaaacccttgcg |
| HMBS (endogenous control) | NM_001024382 | agcttgctcgcatacagacg | agctccttggtaaacaggctt |