Table 1. Oligonucleotide Primers Used for cagA and vacA Typing.
Primer Sequence Annealing Size of Designation (5′ to 3′) 0C Product (bp) |
---|
cagA ACCCCTAGTCGGTAATGGG 49 508 |
GCTTTAGCTTCTGAYACYGC |
vacA s1 ATGGAAATACAACAAACACAC 48 259 |
CTGCTTGAATGCGCCAAAC |
vacA s1a GTCAGCATCACACCGCAAC 54 190 |
CTGCTTGAATGCGCCAAAC |
vacA s1b AGCGCCATACCGCAAGAG 55 187 |
CTGCTTGAATGCGCCAAAC |
vacA s1c CTCTCGCTTTAGTGGGGYT 52 213 |
CTGCTTGAATGCGCCAAAC |
vacA s2 CCTAACACGCCAAATGATCC 54 199 |
CTGCTTGAATGCGCCAAAC |
vacA m1a GGTCAAAATGCGGTCATGG 48 290 |
CCATTGGTACCTGTAGAAAC |
vacA m1b GGCCCCAATGCAGTCATGGAT 56 291 |
CTGTTAGTGCCTAAAGAAGCAT |
vacA m2 GGAGCCCCAGGAAACATTG 50 352 |
CATAACTAGCGCCTTGCAC |
16s rRNA CTGGAGAGACTAAGCCCTCC 49 446 |
AGGATCAAGGTTTAAGGATT |