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. 2014 Nov 5;16(11):e19173. doi: 10.5812/ircmj.19173

Table 1. Oligonucleotide Primers Used for cagA and vacA Typing.

Primer Sequence Annealing Size of Designation (5′ to 3′) 0C Product (bp)
cagA ACCCCTAGTCGGTAATGGG 49 508
GCTTTAGCTTCTGAYACYGC
vacA s1 ATGGAAATACAACAAACACAC 48 259
CTGCTTGAATGCGCCAAAC
vacA s1a GTCAGCATCACACCGCAAC 54 190
CTGCTTGAATGCGCCAAAC
vacA s1b AGCGCCATACCGCAAGAG 55 187
CTGCTTGAATGCGCCAAAC
vacA s1c CTCTCGCTTTAGTGGGGYT 52 213
CTGCTTGAATGCGCCAAAC
vacA s2 CCTAACACGCCAAATGATCC 54 199
CTGCTTGAATGCGCCAAAC
vacA m1a GGTCAAAATGCGGTCATGG 48 290
CCATTGGTACCTGTAGAAAC
vacA m1b GGCCCCAATGCAGTCATGGAT 56 291
CTGTTAGTGCCTAAAGAAGCAT
vacA m2 GGAGCCCCAGGAAACATTG 50 352
CATAACTAGCGCCTTGCAC
16s rRNA CTGGAGAGACTAAGCCCTCC 49 446
AGGATCAAGGTTTAAGGATT