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. Author manuscript; available in PMC: 2015 Feb 16.
Published in final edited form as: Curr Biol. 2009 Dec 3;19(24):2133–2139. doi: 10.1016/j.cub.2009.10.074

Figure 2. Cardiolipin mutant mitochondria are impaired in precursor accumulation at the outer membrane.

Figure 2

(A) Steady-state protein levels. Mitochondria (Mitoch., μg protein) from wild-type (WT), crd1Δ and taz1Δ yeast grown at 21°C in liquid YPG were analyzed by SDS-PAGE and immunoblotting.

(B) Isolated mitochondria were analyzed by blue native electrophoresis and immunoblotting. The band at ~230 kDa in the Tom40 Western blot is only visible after long exposure.

(C) 2D-analysis. Isolated mitochondria were separated by blue native electrophoresis, followed by SDS-PAGE and immunoblotting.

(D) Oxa1 was imported into isolated BY mitochondria at 24°C in the absence of a Δψ. Samples were analyzed by blue native electrophoresis and autoradiography.