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. Author manuscript; available in PMC: 2015 Feb 16.
Published in final edited form as: Curr Biol. 2009 Dec 3;19(24):2133–2139. doi: 10.1016/j.cub.2009.10.074

Figure 4. Assembly of outer membrane proteins in Barth syndrome mitochondria.

Figure 4

(A–C) Mitochondria isolated from control or Barth syndrome patient lymphoblasts were incubated at 37°C (min) in the presence of [35S]VDAC1 or [35S]Tom22. Following import, samples were analyzed by blue native electrophoresis (upper panel) and SDS-PAGE (lower panel) and autoradiography. A sample of reticulocyte lysate (representing 50% of added precursor/import) is also shown on the SDS-PAGE gel. For B, assembled VDAC1 was quantified from four independent experiments (three for 2.5 min). Data are represented as mean +/− SEM.

(D) Control and Barth syndrome mitochondria were analyzed by SDS-PAGE and immunoblotting. SDHA, 70 kDa subunit of succinate dehydrogenase.