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. Author manuscript; available in PMC: 2015 Feb 16.
Published in final edited form as: Cell Rep. 2014 Jan 9;6(2):366–376. doi: 10.1016/j.celrep.2013.12.029

Figure 4. Stat5 in Mast Cells Regulates Der f/SEB-Induced Dermatitis.

Figure 4

Dermatitis was induced with Der f/SEB in MCΔStat5 mice [CKO or Cre(+)] and their floxed control [fl/fl or Cre(−)] mice. *p < 0.05, ***p < 0.001 by Student’s t test.

(A) Western blot analysis of Stat5 in mast cells derived from neonatal skin of MCΔStat5 (CKO) and control (fl/fl) mice.

(B) AD scores accumulated from four separate experiments using three to five mice per group.

(C) Thicknesses of epidermis and dermis after Der f/SEB (D/B) treatment.

(D) Histologic analysis of Der f/SEB-induced dermatitis. Data represent mean ± SEM.

(E and F) Skin sections of naive and Der f/SEB-induced (6 hr after fourth induction) dermatitis in WT mice were stained for phospho-Stat5, mMCP4, and mMCP6. Arrowheads indicate pStat5-positive mast cells. Ep, epidermis; D, dermis; HF, hair follicle. Representative images of mast cells from three experiments are shown in (E) and (F). See also Figure S4.