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. 2015 Feb 6;115:143–156. doi: 10.1016/j.jprot.2014.12.006

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© 2014 The Authors. Published by Elsevier B.V.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2 — (A) Identification of the bait proteinsPI5P4K2β (JPR3 cell line) using Flag MAb (i) and coomassie (ii) and FANCC using αProtein A (iii) and rabbit αFANCG (iv) and Coomassie (v) in parallel pulldowns compared to control DT40 eluates (two right hand lanes). (B) PI5P4K2peptides and (C) Fanconi peptides identified by mass spectrometry. (see Supplemental Tables S2A and B for all peptide sequences).

(A) Identification of the bait proteinsPI5P4K2β (JPR3 cell line) using FLAG MAb (i) and coomassie (ii) and FANCC using αProtein A (iii) and rabbit αFANCG (iv) and coomassie (v) in parallel pull-downs compared to control DT40 eluates (two right hand lanes). (B) PI5P4K2 peptides and (C) Fanconi peptides identified by mass spectrometry. (see Supplemental Tables S2A and B for all peptide sequences).