Table 1. Values of the Time Constants (τ_i) and Normalized (to 1) Pre-Exponential Factors (A_i) of the Multi-Exponential Function Fitting the ps-Emission Transients of CatchER in Various Solvents^a.

in H2O		τ1 (ns)	A1	τ2 (ns)	A2	τ3 (ns)	A3	τave (ns)
Ex372; Em440	Apo	0.15 ± 0.01	0.81 ± 0.01	0.37 ± 0.01	0.19 ± 0.01	NA	NA	0.19 ± 0.01
	Holo	0.17 ± 0.01	0.81 ± 0.01	0.41 ± 0.01	0.19 ± 0.01	NA	NA	0.22 ± 0.01
Ex372; Em510	Apo	0.16 ± 0.03	0.47 ± 0.03	0.73 ± 0.24	0.09 ± 0.04	3.14 ± 0.03	0.44 ± 0.02	1.51 ± 0.10
	Holo	0.21 ± 0.01	0.37 ± 0.02	3.34 ± 0.03	0.63 ± 0.02	NA	NA	2.18 ± 0.06
Ex467; Em510	Apo	2.01 ± 0.14	0.75 ± 0.11	4.47 ± 0.87	0.25 ± 0.11	NA	NA	2.61 ± 0.06
	Holo	2.09 ± 0.03	0.77 ± 0.01	4.73 ± 0.10	0.23 ± 0.01	NA	NA	2.54 ± 0.19

in D2O		τ1 (ns)	A1	τ2 (ns)	A2	τ3 (ns)	A3	τave (ns)
Ex372; Em440	Apo	0.12 ± 0.01	0.79 ± 0.08	0.31 ± 0.06	0.21 ± 0.06	NA	NA	0.16 ± 0.01
	Holo	0.14 ± 0.01	0.87 ± 0.01	0.40 ± 0.01	0.13 ± 0.01	NA	NA	0.17 ± 0.01
Ex372; Em510	Apo	0.10 ± 0.02	0.67 ± 0.07	0.39 ± 0.10	0.17 ± 0.07	3.06 ± 0.04	0.15 ± 0.01	0.61 ± 0.03
	Holo	0.13 ± 0.01	0.60 ± 0.04	0.41 ± 0.10	0.15 ± 0.05	3.42 ± 0.01	0.25 ± 0.01	0.99 ± 0.05
Ex467; Em510	Apo	2.18 ± 0.24	0.81 ± 0.01	5.24 ± 0.10	0.19 ± 0.02	NA	NA	2.55 ± 0.16
	Holo	1.81 ± 0.03	0.56 ± 0.16	3.62 ± 0.46	0.44 ± 0.16	NA	NA	2.60 ± 0.07

^aThe amplitude-weighted average lifetimes of τ_ave were calculated as [τ_ave = ∑_i = 1ⁿ(A_iτ_i/∑_i = 1A_i)].