Real-time RT-PCR was performed on mRNA isolated from whole colonic serial sections for (A) Reg3β, Reg3γ, SOCS3 (B) IL-6, IL-10, IL-17A (C) IFN-γ, IL-4, IL-1β, and TNF-α expression in Stat3Flx/Flx littermate controls and Stat3ΔIEC mice at day 0, day 7, and day 28 (n>5 mice per genotype). Data are normalized to β-actin mRNA expression and expressed as fold change to Stat3Flx/Flx day 0 littermate controls shown as mean + SEM. Differences between genotypes at day 0 and day 28 were compared by unpaired t-test (*, p < .05; ** p < .005; *** p < .0001).