Figure 2.

IK8L increased AM viability in Kp-infected mice.

Notes: (A) Cell viability was determined in AMs by MTT assay. AM cells were obtained separately from infected mice and IK8L-treated mice. The absorbance of each sample was recorded at 560 nm to determine the cell viability rate. (B) Scattered distribution graph of apoptotic staining of the AM cells with or without IK8L treatment. The data of apoptotic percentage of treated cells vs the control are representative of two experiments (arrows indicating the apoptotic levels). (C and D) PMN infiltration in the BAL and blood was counted by Hema staining (Thermo Fisher, Waltham, MA, USA). (E) Superoxide production in AM cells detected using an NBT assay. (F) Oxidative stress in AM cells was determined by H₂DCF assay. Data are shown as mean ± SEM of n=3 samples and are representative of three independent experiments. **P<0.01; ***P<0.001; one-way ANOVA (Tukey’s post hoc).

Abbreviations: AM, alveolar macrophage; ANOVA, analysis of variance; BAL, bronchoalveolar lavage; h, hours; H₂DCF, dihydrodichlorofluorescein diacetate; Kp, Klebsiella pneumoniae; MTT, 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyltetrazolium bromide; NBT, nitroblue tetrazolium; PMN, polymorphonuclear neutrophil; Q, quarter; RFU, relative fluorescence units; RLU, relative LUC units; SEM, standard error of the mean.