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. 2015 Feb 9;10:1045–1059. doi: 10.2147/IJN.S73303

Figure 5.

Figure 5

IK8L modulated Kp-induced inflammatory reaction via the IL-6/JAK/STAT3 signaling pathway.

Notes: (A–C) ELISA was used to measure IL-6, TNF-α and IL-1β in BAL fluids of mice 8 hours and 24 hours post infection, respectively. (D) Cell signaling proteins relating to the JAK2/STAT3 signaling pathway were evaluated in lung tissue by Western blotting analysis. (E) Expression and phosphorylation of signaling proteins relating to NF-κB transcription factor were evaluated in lung tissue by Western blotting. (F) Cell signaling proteins relating to the AKT signaling pathway were evaluated by Western blot. β-actin was used as a loading control. Data are shown as mean ± SEM of n=3 mice per group and are representative of three independent experiments. *P<0.05; **P<0.01; ***P<0.001; one-way ANOVA (Tukey’s post hoc).

Abbreviations: ANOVA, analysis of variance; BAL, bronchoalveolar lavage; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; Kp, Klebsiella pneumoniae; SEM, standard error of the mean; TNF-α, tumor necrosis factor alpha; p, phosphorylation.