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. 2015 Jan 27;4(1):e001423. doi: 10.1161/JAHA.114.001423

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© 2015 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Figure 2. — Schematic view of the genomic structure and CpG dinucleotides selected for analysis in the Toll‐like receptor 2 (TLR2) gene. The chromosomal location of the PCR amplicon for the TLR2 gene pyrosequencing assay is based on the Human Genome Assembly 2009 (GRC37/hg19) (UCSC Genome Browser). The CpG island, gene promoter region, PSQ CpG sites, PCR primer, and PSQ sequence primer location are shown in the figure. TSS indicates the transcription start site; PCR, polymerase chain reaction; PSQ, pyrosequencing. Position 1: CpG154605258; Position 2: CpG154605262; Position 3: CpG154605264; Position 4: CpG154605272; Position 5: CpG154605276.