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High-magnification fluorescent staining images of chondroitin sulfate proteoglycans (CSPGs) and hyaluronidase-4 (Hyal-4) at 3 weeks after hemisection. Photomicrographs of the lesioned tissue 3 weeks after the hemisection. The results of immunofluorescence, stained for CS56 (CSPGs) (green) and Hyal-4 (red), are shown. The Hyal-4-immunostained area surrounds the CS56-positively stained area. In some parts, Hyal-4 staining invaded the area stained for CS56. Scale bar=50 µm.
