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. 2014 Dec 23;156(3):1033–1039. doi: 10.1210/en.2014-1707

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Figure 2. — TSPO deficiency does not affect progesterone production in MA-10 cells. A, TSPO deletion in the three MA-10:TspoΔ/Δ clones did not affect baseline or Bt₂cAMP-stimulated progesterone production. Bt₂cAMP stimulation increased progesterone levels to a similar extent in both intact MA-10:Tspo+/+, and TSPO-deleted clones: MA-10:TspoΔ/Δ1, MA-10:TspoΔ/Δ2, and MA-10:TspoΔ/Δ3. Progesterone levels after Bt₂cAMP treatment in clone MA-10:TspoΔ/Δ3 were significantly higher than MA-10:Tspo+/+ cells. Mean values were generated from two to three experiments conducted in triplicate. B, Western blots showing absence of TSPO and the induction of STAR after Bt₂cAMP stimulation of MA-10:TspoΔ/Δ clones and MA-10:Tspo+/+ cells. Induction of STAR expression after Bt₂cAMP stimulation were identical in the different MA-10:TspoΔ/Δ clones compared with MA-10:Tspo+/+ cells. Control bands are β-Actin (ACTB).