Figure 2.

Identification of miR-21 KO clones and elimination of marker genes. (A) Detection of miR-21 level by qRT-PCR. Over a 1000-fold reduction was detected in all of the five miR-21 KO clones with P value <0.01 for all five clones. (B) Excision of marker genes in miR-21 KO clone #1 cells by Cre. Shown here are representative images. (C) Both miR-21 KO clones #1 and #2 carry distinct PCR products using primers miR-21–5.1 and miR-21–3.1. (D) DNA sequences for each of the PCR products from clones #1 and #2. Both clones revealed identical sequences. Insertion sequences are shown in blue and loxP sequences are in red.