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. 2014 Nov 20;43(3):e17. doi: 10.1093/nar/gku1198

Figure 3.

Figure 3.

Characterization of miR-21 KO clones in HCT-116. (A) Detection of miR-21 in miR-21 KO clones by qRT-PCR; P value <0.01 for all seven clones compared to vector control. miR-21 KO #2 and #17 (in green) were selected for further analysis. (B) Detection of alterations at the target region of miR-21 KO #2 and #17 by DNA sequencing. (C) miR-21 knockout suppresses cell growth, as determined by colony formation assays. (D) miR-21 knockout increases the miR-21 target GAS5, as detected by qRT-PCR. (E) miR-21 knockout increases the miR-21 target PDCD4, as detected by western blot. Values in (C) and (D) are means of ± SE (n = 3). *P < 0.05 and **P < 0.01.